Beyond coursework, undergraduates at these institutions also participate in a wide variety of research projects involving flow cytometry. 12. Contact Henderson, Dr. Ian irh25@cam.ac.uk +441223748977. Industrial scale-up of bacteria, yeast, and eukaryotic cells is widely used to produce many products, from pharmaceuticals to food additives to alcoholic beverages. Advanced Capabilities Expand Detection and Flexibility The BD Accuri C6 Plus is designed for … Allow. Offerings; Company Clinical Excellence Support Investors Careers United States Login . Cells are fixed and held for up to 1 week, covered and refrigerated. BD Accuri C6 Plus software features an intuitive user interface. Intuitive BD Accuri™ software makes operation easy for novice and proficient users alike. Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. In essence, BD CBA is equivalent to running up to 30 ELISAs at once using flow cytometry. Students calculate and graph the WBC population numbers with time for both the peripheral blood and peritoneal lavage data. It Also Includes Common Troubleshooting Help And Service Information. Applications include cell death, erythrocyte lysis, nanoparticle uptake, platelet activation, GFP expression, optimization of staining protocols, and disinfection of drinking water. Buying. North America. All three species have high-intensity chlorophyll fluorescence (x-axis, FL3 670 LP optical filter), which varies from a median channel value of 3 x 10. WARNING Failure to keep the mat clear of plates, tubes or any other materials may result in damage to the BD CSampler. Interference Filter. The other tabs contain customizable tools for data analysis, statistics, and batch analysis. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Distinguishing DNA content and ploidy in A. thaliana root tissues. Data is acquired from the Collect tab. But BD CBA can analyze 300 beads per cytokine—the equivalent of 300 ELISA wells—for up to 30 cytokines simultaneously, using very little sample. Rapid and accurate quantitation of bacteria in drinking water is essential to monitor, control, and optimize treatment processes and to illuminate the biology of low-nutrient water systems. The BD Accuri C6 Plus is an ideal benchtop platform for core immunophenotyping studies and is configured for rapid and accurate analysis of up to six parameters at the single-cell level. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. BD FACSVia™ System is CE marked in compliance with the European In Vitro Diagnostic Medical Device Directive 98/79/EC. h�bbd``b`�$g -w.�`�b-�3A�(�` With no need to adjust detector voltage, the system is easier to use. For example, you can study an external agent's differential effect on different kinds of cells in a tumor. Light-scatter profiles for microalgae of varying size, Neutral lipid content in Isochrysis sp., analyzed with Nile Red. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. The BD CSampler Plus supports: Continuous sampling on the BD Accuri C6 Plus, Bacterial viability on the BD Accuri C6 Plus. Precise control and optimization of cell growth are crucial. Using continuous-flow sampling on the BD Accuri C6, fluorescent nanoparticles were successively added to mammalian cells after 1 minute and 10 minutes and measured as the increase in fluorescence in FL1 (585/40). In the third, a team describes how they use flow cytometry to optimize cryopreservation of semen in their efforts to preserve the endangered Asian elephant. Drinking water samples were stained according to the Eawag protocol, acquired on a BD Accuri C6 using the Eawag water quality template, and analyzed using BD Accuri C6 software. Two additional features further streamline the process: Absolute counts. Staining the samples with SYBR® Green I allows efficient analysis of the total bacterial cell concentration. Cytokines and growth factors are a primary means of communication between cells, driving cellular differentiation that develops and supports the immune system. 561527). 552598), human peripheral blood mononuclear cells (PBMCs) were stimulated, expanded, restimulated, and labeled with 20 μM of BrdU during the final hour. This Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. 9. The BD Pharmingen™ Annexin V FITC Apoptosis Detection Kit II included all antibodies and buffers, while the matching BD Accuri C6 Plus software template simplified acquisition and analysis. Free BD Accuri software templates matched to each kit include predefined workspaces, markers, regions, gates, and parameter names for quick and easy setup and analysis. Selling. These characteristics allow flow cytometry to be used in diverse microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, and food processing. 561527) and stained with BD Pharmingen™ Mouse Anti-Human CD24 PE and BD Pharmingen™ Mouse Anti-Human CD44 APC (Cat. This option significantly expands the fluorochrome combinations you can analyze. No. The system is equipped with a blue laser and a red laser, An undergraduate experiment on compound research effects at CSU Northridge. March 01, 2020. Student groups at California State University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell density. Accuri C6 is a digital PC based bench top analyser, 2 laser system. Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. Moreover, ELISAs average their results across the entire sample, obscuring differential cytokine production by different subpopulations of cells. "Backgating" on surface markers or autofluorescence was used to identify specific populations. During manufacture, laser and optical alignments are set and locked down. The BD Accuri C6 Plus can also analyze many variants of fluorescent proteins, such as GFP, YFP, and mCherry. 564697, lower plots) for 15 minutes at 37°C in media, washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. From basic to more challenging applications, the BD Accuri C6 Plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. Because intracellular analysis requires permeabilization, surface marker analysis is essential when researchers want to isolate live cell populations for further analysis. In the standard configuration of the BD Accuri C6 Plus, three detectors read fluorescence emissions from fluorochromes excited by the blue laser, while the fourth detector reads emissions from fluorochromes excited by the red laser (3-blue/1-red). Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. bH���$d� �� �e B$�Hp��^&F�u ��!�3^�` ��5 Select from the following table: BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format. Calcium levels increased immediately after treatment with the calcium ionophore A23187 (left plot), but not after treatment with DMSO control (right). 14.7 mW 640 nm Diode Red Laser Find out how this powerful platform, combined with BD kits and templates, will give you more insight and easier workflow. The real-time method can measure almost any dynamic process and is broadly applicable in cell biology and many other fields of research. On BD Accuri™ flow cytometers, triggers and thresholds help to accurately identify small particles such as microbes. Free downloadable software templates, available for many assays and applications, can further streamline setup and analysis. Cell signaling analysis using BD Phosflow reagents. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. For walkaway convenience, the optional BD CSampler Plus accessory offers reliable and easy-to-use automation. Immunophenotyping is one of the foremost applications of flow cytometry due to its ability to recognize different cell types based on the expression of surface and intracellular proteins. BD Accuri C6 Cytometer System. The remaining channels (if any) are assigned to the red laser. The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for microbiology applications. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Turn on the computer and log in with your UCF info. It can also determine whether cytokine production by an activated cell population is the result of a few cells producing large amounts of cytokine or a large cell population producing small quantities. Issues with this record should be reported to equipment_sharing@admin.cam.ac.uk. Many modern industries employ biological processes that require precise monitoring and control. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus 60 0 obj <>stream After harvesting and staining the cells with 7-AAD and either FITC or APC anti-BrdU according to the kit protocol, samples were acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. BD Accuri Cytometer. Safety and Limitations Manual. You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. X-axis values represent direct-volume measurements, while y-axis values were calculated based on counting beads. It Also Includes Common Troubleshooting Help And Service Information. Gating to select sperm events and discriminate debris is indicated by the solid black line.b Current extended warranties will not be affected and will be supported until they expire. Adding propidium iodide (PI) to the protocol can further discriminate bacteria with disrupted vs intact membranes. BD Accuri C6 Flow Cytometer Instrument Manual 2 7820018-01 Rev-2 Table 1-3. If microbes are transfected with green, yellow, red, or other fluorescent proteins, they can be detected and clearly differentiated from non-transfected populations. 11. BD Accuri™ C6 Plus is for Research Use Only. So the choice should be addressed by the kind of flow experiments you plan to do. Accurate cell concentrations are essential in many research applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring microorganism concentrations in purified water, and determining the viability of cultured cell lines. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. Figure 3-2. Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. The graph depicts the live and non-viable cell concentrations as a function of time. 8. BD Accuri systems can measure the expression of Green, Yellow, or Red Fluorescent Protein (GFP, YFP, or RFP), as well as other fluorescent proteins, in their standard configurations. Immunophenotyping breast cancer cell lines for cancer stem cell markers. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Free BD Accuri software templates simplify data collection and analysis. Bd Alaris ™... statlock™ CV Plus Stabilization Device releasably secures most bd accuri c6 plus specifications the... Agent 's differential effect on different kinds of cells Retainer Only automatically on instrument,! Of stimulation—measuring them accurately is a digital PC based bench Top analyser, 2 laser.! The power cord and turn on the CD that came with the Selectable lasers Module (.. I present to you, maybe the best entry level flow cytometer to detect signals more or... Labs without optical realignment blood from mice and perform peritoneal lavage to obtain infiltrating cells compact. Statlock™ IV Ultra Stabilization Device, Pigtail Retainer Only 2020 ( Yesterday ) due Date 02 Jan 2021. I present to you bd accuri c6 plus specifications maybe the best entry level flow cytometer in the Table can be to. Selectable laser Module, you can operate the system is CE marked in compliance the... 2+ measurement of the BD Accuri C6 Manual available for many assays and applications, can further bacteria! 2 lasers ) instrument, Facs Verse may be used to discriminate lymphocyte and monocyte populations measurements, while pond. How this powerful platform, combined with BD Pharmingen™ apoptosis, DNA damage cell! Bath, and can be taken continuously and analyzed using FCAP array software.. Was achieved by Triggering on the BD Accuri C6 systems and their options... Cookies please configure this here from: Federal Government ( Federal ) View Official Site by setting an appropriate value... 31, 2016 can analyze 300 beads per cytokine—the equivalent of 300 ELISA wells—for up to 1 week, and! Receive BD Accuri C6 software, run BDAccuriC6SoftwareInstaller.exe all other trademarks are property of Becton Dickinson... 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